100mm Cell Culture Insert With ISO9001&13485 certified, 100mm Cell Culture Inserts are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials. Nest is committed to be your best choice for laboratory and medical consumables.
Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, molecular biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments
Product Features：
Innovative hanging design facilitates pipetting.
Supplied in multiple well plates: 6-well, 12-well, 24well.
PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
Transparent PET membrane is easy to observe.
The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
Compatible with most solvents used for fixing and dyeing.
Application：
Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and
independent cell lines.
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out
metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.
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packing specification
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Inserts/plate
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Insert Diameter(mm)
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Well Volume(ml)
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Insert Volume(ml)
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Insert Membrane Growth Area（cm²）
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100mm dish
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1 inserts
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75
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13
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9
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44
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Pore Size(um)
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Membrane Density（well/cm²）
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0.4
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1x10^8
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3.0
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2x10^6
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5.0
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4x10^5
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8.0
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1x10^5
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50% Transparent PC Membrane Cell Insert, Tissue Culture Treated(Individually packaged in peelable Tyvek film and Blister，10/case)
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Cat.No.
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Type（Well)
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Pore Size(um)
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726001
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100mm dish
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3.0
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Attention：
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.
Guidelines for us：
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
Company Introduction
Found in 2009,Wuxi NEST Biotechnology is a worldwide manufacturer of plastic laboratory products. State-of-the-art manufacturing equipment is used on an expansive manufacturing site based in Wuxi, China. Decades of plastic-injection molding experience allows us to maintain the highest quality and performance standards expected by the hospitals, research institutions and indusrial/clinical laboraories that we serve, NEST is your best choice for laboratory consumables.
NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. With stricty controlled ISO 8 grade clean rooms, exacting manufacturing standards and rigid QA procedures, the NEST brand enjoys an excellent reputation nationwide and abroad. NEST products have been exported to North America, Europe, Japan, Korea, India and other countries.
NEST is committed to innovation. We continue to research and develop new platic consumables suitable for life sciences research so that we can provide more cost-effective solutions and cutting-edge laboratory consumables for our end-users.
NEST has continually positioned itself as a brand for high end laboratory consumables.
Our Services & Strength
NEST boasts of a high power laminar flow clean room meeting the ISO 8 grade with a floor space of 2,000 square meters. These facilities are equipped with a central feeding system, high precision injection machinery from Japan, robot arms with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment machines from Belgium, Germany and USA. Inner room moisture content and temperature are adjustable. In 2013, NEST installed a Rhodotron TT 200 from Belgium IBA, a world leader in the E-Beam sterilization industry. NEST completes a full process chain from the sourcing of the raw materials to the in-house sterilizationof the final products ready for delivery to clients.
NEST’s manufacturing site has been certified with an ISO 9001:2008 management system. Our sterilization procedures meet the ISO 11137:2006 and ISO 13485 standards.
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